In molecular biology, one of the most elementary techniques is the PCR : Polymerase Chain Reaction
The PCR method (Polymerase Chain Reaction) is used to amplify in vitro a specific area within a nucleic acid in order to obtain a sufficient quantity to detect and study it.
The PCR reactions are composed of several 'PCR cycles' enabling the replication of a double-stranded DNA matrix. Thus, the PCR products obtained at the end of each cycle are used as matrix for the next cycle. The amplification process is therefore exponential.
The PCR proceeds in three stages:
1. Denaturation : separation of the “”double-stranded”" matrix in “”single-stranded”"
2. Partitionning : to target the DNA region to amplify with the help of specific PCR primers.
3. PCR amplification : stage of the complementary strand polymerization. At the end of each cycle, double-stranded DNA is synthesized.
The instruments used for PCR are the 'thermocylers'. HTDS proposes you a full range of solutions and consumables for your PCR.