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In molecular biology, one of the most elementary techniques is the PCR : Polymerase Chain Reaction The PCR method (Polymerase Chain Reaction) is used to amplify in vitro a specific area within a nucleic acid in order to obtain a sufficient quantity to detect and study it. The PCR reactions are composed of several 'PCR cycles' enabling the replication of a double-stranded DNA matrix. Thus, the PCR products obtained at the end of each cycle are used as matrix for the next cycle. The amplification process is therefore exponential. The PCR proceeds in three stages: 1. Denaturation : separation of the ""double-stranded"" matrix in ""single-stranded"" 2. Partitionning : to target the DNA region to amplify with the help of specific PCR primers. 3. PCR amplification : stage of the complementary strand polymerization. At the end of each cycle, double-stranded DNA is synthesized. The instruments used for PCR are the 'thermocylers'. HTDS proposes you a full range of solutions and consumables for your PCR.
The DNA sequencing consists in determinating the chainning order of nucleotides in a DNA sample. Currently, most of DNA sequencing are realized with the chain termination method. This technique uses the reaction of the DNA polymerization with the help of a polymerase DNA and of didesoxyribonucleotides (ddNTP) with fluorescent markers. A marker / a color representing a base (dNTP). The DNA sequence contains the information necessary for living being to survive and reproduce. Determinate this sequence is therefore useful for researches aiming to study how organisms are living, as well as for subjects to put into practice. In medicine, it can be used to identify, diagnose and potentially find treatments for genetic diseases. HTDS proposes you a full range of solutions and consumables for DNA sequencing.