In molecular biology, one of the most elementary techniques is the PCR : Polymerase Chain Reaction The PCR method (Polymerase Chain Reaction) is used to amplify in vitro a specific area within a nucleic acid in order to obtain a sufficient quantity to detect and study it. The PCR reactions are composed of several ‘PCR cycles’ enabling the replication of a double-stranded DNA matrix. Thus, the PCR products obtained at the end of each cycle are used as matrix for the next cycle. The amplification process is therefore exponential. The PCR proceeds in three stages: 1. Denaturation : separation of the “”double-stranded”” matrix in “”single-stranded”” 2. Partitionning : to target the DNA region to amplify with the help of specific PCR primers. 3. PCR amplification : stage of the complementary strand polymerization. At the end of each cycle, double-stranded DNA is synthesized. The instruments used for PCR are the ‘thermocylers’. HTDS proposes you a full range of solutions and consumables for your PCR.
Thermal Cycler (PCR)
Thermal cycler (Also known as PCR machine) is an equippment which automates the 'classical' PCR reaction. A thermal cycler is made up with a thermal block (""thermal Peltier block"") with 'holes/wells"" where one can insert tubes containing the PCR reactive mix. HTDS represents the brand Applied Biosystems and its range of thermocyclers for Algeria and Tunisia :
The acronym RT-PCR stands for Reverse Transcriptase PCR, ie a PCR after an inverse transcription of a ribonucleic acid (RNA) in complementary DNA (cDNA). In reality, the RT-PCR is a 'classical"" PCR realized on a complementary DNA (or cDNA). The RT-PCR has been developed to use the RNA as an amplification matrix of the PCR. It is certainly the most sensible method to detect (and possibly quantify, qRT-PCR) messengers RNA at the level of an organ, a tissue or a cell , on the occasion of gene expression studies. Applied Biosystems , High Capacity cDNA Reverse Transcription Kit : This product (kit) contains all the necessary reactifs for quantitative conversion of total RNA in cDNA (1strand) in reaction volumes of 20 µL. Dynamic range: 10ng-2µg ARN Total. > Included in the kit : MultiscribeTM Reverse Transcriptase, dNTPs (100mM), 10X random hexamers, 10X buffers RT, Rnase Inhibitor (optional), etc. > Available sizes : 200 / 1000 reactions, with/without RNA Inhibitor. To receive more informations on these products, please make contact with your HTDS local representant in Algeria and Tunisia.
PCR and qPCR consumables
In addition to the instrumentation part, we also offer a full range of c to ensure the proper functioning of your genomic platforms. At each amplification cycle, the amount of total DNA or amplicon is measured by means of a fluorescent marker. Obtaining the complete kinetics of the polymerization reaction makes it possible to obtain an absolute quantification of the initial amount of target or relative DNA (relative to other targets). The real-time PCR technique is used in a multitude of applications: development of primers, quantification (gene expression), detection of point mutations, assay of GMOs in products for human consumption, specific detection and sensitive pathogens of veterinary interest - quantification of bacterial, viral or parasitic load, etc ... Just like the range of thermocyclers, we represent the Thermo Scientific brand for real-time PCR instruments. Plastic Consumables: Tubes, plate, caps, adhesives ...