The DNA sequencing consists in determinating the chainning order of nucleotides in a DNA sample. Currently, most of DNA sequencing are realized with the chain termination method. This technique uses the reaction of the DNA polymerization with the help of a polymerase DNA and of didesoxyribonucleotides (ddNTP) with fluorescent markers. A marker / a color representing a base (dNTP). The DNA sequence contains the information necessary for living being to survive and reproduce. Determinate this sequence is therefore useful for researches aiming to study how organisms are living, as well as for subjects to put into practice. In medicine, it can be used to identify, diagnose and potentially find treatments for genetic diseases. HTDS proposes you a full range of solutions and consumables for DNA sequencing.
HTDS distributes DNA sequencers based on capillary electrophoresis technology to separate DNA fragments. The proposed solutions have a laser to detect successive fluorescent emissions of fragments during their migration within capillaries. This enables oligonucleotide sequencing. Solutions displayed below are recognized as major sequencing instrumentation references in the genetic analysis world. According to your applications and the flow you need, we propose a range of instruments adapted to your specifications.
DNA sequencing consumables
In addition to our instrumentation range, we distribute a whole range of consumables and softwares to offer you full sequencing solutions. Our range of consumables is made of ready-made kits / ready mix (incl. ddNTPs), buffers, TaqPolymerase®, but also primers, purification solutions, POP4/6/7 (Performance Optimised Polymers), plastics, etc. Concerning data treatment, we have different softwares for : > Sequences analysis (with or without reference) : mutation detection, etc. Applied Biosystems, Variant ReporterTM Software v1.0 This software enables to compare sequences with or without initial references, to detect and analyse mutations, to discover and validate the presence of SNPs and confirm sequences. To receive more informations on this product, please contact your HTDS local representative in Tunisia or Algeria. > Fragments analysis: microsatellites genotyping, human identification, SNP genotyping, etc. GeneMapper is a sequencing software using fragment analysis. It allows to realize applications such as AFLP, LOH (loss of heterozygosity), microsatellite, SNPs genotyping, etc. It also includes the SNPlexTM technology (high-speed genotyping) for the simultaneous analysis of 48 SNPs per sample. This product is compatible with the whole ABI range of capillary electrophoresis sequencers. To receive more details on this product, please contact your HTDS local representative.